Comprehensive studies on optimization of ligno-hemicellulolytic enzymes by indigenous white rot hymenomycetes under solid-state cultivation using agro-industrial wastes.
Identifieur interne : 000165 ( Main/Exploration ); précédent : 000164; suivant : 000166Comprehensive studies on optimization of ligno-hemicellulolytic enzymes by indigenous white rot hymenomycetes under solid-state cultivation using agro-industrial wastes.
Auteurs : Yuvarani Naidu [Malaisie] ; Yasmeen Siddiqui [Malaisie] ; Abu Seman Idris [Malaisie]Source :
- Journal of environmental management [ 1095-8630 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Industrial Waste, Laccase.
- Fermentation, Polyporaceae, Trametes.
Abstract
The disposal of oil palm biomass is a huge challenge in Malaysian oil palm plantations. The aim of this study was to develop efficient solid-state cultivated (SSC) ligno-hemicellulolytic bio-degrader formulations of indigenous white-rot hymenomycetes (Trametes lactinea FBW and Pycnoporus sanguineus FBR) utilizing oil palm empty fruit bunches (EFB), rubber wood sawdust (SD) and vermiculite (V) either alone or in combination as substrates. Based on significant laccase (849.40 U mg-1 protein), xylanase (42.26 U g-1 protein) and amylase (157.49 U g-1 protein) production, SD+V (T5) and V (T3) were the optimum substrates for SSC of T. lactinea FBW. Whereas, utilizing EFB (T1) substrate for SSC of P. sanguineus FBR enhanced the production of MnP (42.51 U mg-1 protein), LiP (103.20 U mg-1 protein) and CMCase (34.39 U g-1 protein), enzymes. Apparently, this is the first study reporting on the protein profiles by T. lactinea FBW, producing two isoforms of un-purified laccase (~55 and 70 kDa) and MnP (~40 and 60 kDa) and a CMCase band (~60 kDa) during SSC on SD+V (T5) substrate. Interestingly, this is also the first report to document a single isoform of un-purified laccase (~50 kDa), MnP (~45 kDa), CMCase (~60 kDa) and xylanase (~55 kDa) by P. sanguineus FBR during SSC on empty fruit bunches substrate. The computed Principal Component Analysis (PCA) Biplot analysis elucidated the relationship between the solid substrate compositions, the hymenomycete strain, ligno-hemicellulolytic enzyme profiles, and cultivation time. Therefore, it is suggested to use PCA as a tool for multivariate analysis method for comprehensive selection and optimization of ligno-hemicellulolytic enzyme cocktails by the indigenous white rot hymenomycetes. These non-toxic (acute oral toxicity) formulations are safe to be used in field applications to efficiently degrade oil palm trunks and root mass that had been felled, chipped or pulverized under zero burning waste management program. This study could also serve as an alternative method for efficient utilization of agro-industrial waste as substrates for the development of cost-effective bio-degraders formulations for agro-waste management.
DOI: 10.1016/j.jenvman.2019.110056
PubMed: 31929034
Affiliations:
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The aim of this study was to develop efficient solid-state cultivated (SSC) ligno-hemicellulolytic bio-degrader formulations of indigenous white-rot hymenomycetes (Trametes lactinea FBW and Pycnoporus sanguineus FBR) utilizing oil palm empty fruit bunches (EFB), rubber wood sawdust (SD) and vermiculite (V) either alone or in combination as substrates. Based on significant laccase (849.40 U mg<sup>-1</sup> protein), xylanase (42.26 U g<sup>-1</sup> protein) and amylase (157.49 U g<sup>-1</sup> protein) production, SD+V (T5) and V (T3) were the optimum substrates for SSC of T. lactinea FBW. Whereas, utilizing EFB (T1) substrate for SSC of P. sanguineus FBR enhanced the production of MnP (42.51 U mg<sup>-1</sup> protein), LiP (103.20 U mg<sup>-1</sup> protein) and CMCase (34.39 U g<sup>-1</sup> protein), enzymes. Apparently, this is the first study reporting on the protein profiles by T. lactinea FBW, producing two isoforms of un-purified laccase (~55 and 70 kDa) and MnP (~40 and 60 kDa) and a CMCase band (~60 kDa) during SSC on SD+V (T5) substrate. Interestingly, this is also the first report to document a single isoform of un-purified laccase (~50 kDa), MnP (~45 kDa), CMCase (~60 kDa) and xylanase (~55 kDa) by P. sanguineus FBR during SSC on empty fruit bunches substrate. The computed Principal Component Analysis (PCA) Biplot analysis elucidated the relationship between the solid substrate compositions, the hymenomycete strain, ligno-hemicellulolytic enzyme profiles, and cultivation time. Therefore, it is suggested to use PCA as a tool for multivariate analysis method for comprehensive selection and optimization of ligno-hemicellulolytic enzyme cocktails by the indigenous white rot hymenomycetes. These non-toxic (acute oral toxicity) formulations are safe to be used in field applications to efficiently degrade oil palm trunks and root mass that had been felled, chipped or pulverized under zero burning waste management program. This study could also serve as an alternative method for efficient utilization of agro-industrial waste as substrates for the development of cost-effective bio-degraders formulations for agro-waste management.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31929034</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-8630</ISSN><JournalIssue CitedMedium="Internet"><Volume>259</Volume><PubDate><Year>2020</Year><Month>Apr</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of environmental management</Title><ISOAbbreviation>J Environ Manage</ISOAbbreviation></Journal><ArticleTitle>Comprehensive studies on optimization of ligno-hemicellulolytic enzymes by indigenous white rot hymenomycetes under solid-state cultivation using agro-industrial wastes.</ArticleTitle><Pagination><MedlinePgn>110056</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0301-4797(19)31774-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jenvman.2019.110056</ELocationID><Abstract><AbstractText>The disposal of oil palm biomass is a huge challenge in Malaysian oil palm plantations. The aim of this study was to develop efficient solid-state cultivated (SSC) ligno-hemicellulolytic bio-degrader formulations of indigenous white-rot hymenomycetes (Trametes lactinea FBW and Pycnoporus sanguineus FBR) utilizing oil palm empty fruit bunches (EFB), rubber wood sawdust (SD) and vermiculite (V) either alone or in combination as substrates. Based on significant laccase (849.40 U mg<sup>-1</sup> protein), xylanase (42.26 U g<sup>-1</sup> protein) and amylase (157.49 U g<sup>-1</sup> protein) production, SD+V (T5) and V (T3) were the optimum substrates for SSC of T. lactinea FBW. Whereas, utilizing EFB (T1) substrate for SSC of P. sanguineus FBR enhanced the production of MnP (42.51 U mg<sup>-1</sup> protein), LiP (103.20 U mg<sup>-1</sup> protein) and CMCase (34.39 U g<sup>-1</sup> protein), enzymes. Apparently, this is the first study reporting on the protein profiles by T. lactinea FBW, producing two isoforms of un-purified laccase (~55 and 70 kDa) and MnP (~40 and 60 kDa) and a CMCase band (~60 kDa) during SSC on SD+V (T5) substrate. Interestingly, this is also the first report to document a single isoform of un-purified laccase (~50 kDa), MnP (~45 kDa), CMCase (~60 kDa) and xylanase (~55 kDa) by P. sanguineus FBR during SSC on empty fruit bunches substrate. The computed Principal Component Analysis (PCA) Biplot analysis elucidated the relationship between the solid substrate compositions, the hymenomycete strain, ligno-hemicellulolytic enzyme profiles, and cultivation time. Therefore, it is suggested to use PCA as a tool for multivariate analysis method for comprehensive selection and optimization of ligno-hemicellulolytic enzyme cocktails by the indigenous white rot hymenomycetes. These non-toxic (acute oral toxicity) formulations are safe to be used in field applications to efficiently degrade oil palm trunks and root mass that had been felled, chipped or pulverized under zero burning waste management program. This study could also serve as an alternative method for efficient utilization of agro-industrial waste as substrates for the development of cost-effective bio-degraders formulations for agro-waste management.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Naidu</LastName><ForeName>Yuvarani</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biology Division, Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia. Electronic address: yuvarani@mpob.gov.my.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Siddiqui</LastName><ForeName>Yasmeen</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Plantation Science and Technology, Institute of Plantation Studies, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia. Electronic address: yasmeen@upm.edu.my.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Idris</LastName><ForeName>Abu Seman</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Biology Division, Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Environ Manage</MedlineTA><NlmUniqueID>0401664</NlmUniqueID><ISSNLinking>0301-4797</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007220">Industrial Waste</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007220" MajorTopicYN="N">Industrial Waste</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011124" MajorTopicYN="Y">Polyporaceae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055454" MajorTopicYN="Y">Trametes</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agro-industrial waste</Keyword><Keyword MajorTopicYN="N">Hydrolytic enzymes</Keyword><Keyword MajorTopicYN="N">Lignocellulosic biomass</Keyword><Keyword MajorTopicYN="N">Oxidative enzymes</Keyword><Keyword MajorTopicYN="N">PCA Biplot</Keyword><Keyword MajorTopicYN="N">Waste management</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>12</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31929034</ArticleId><ArticleId IdType="pii">S0301-4797(19)31774-8</ArticleId><ArticleId IdType="doi">10.1016/j.jenvman.2019.110056</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Malaisie</li></country></list><tree><country name="Malaisie"><noRegion><name sortKey="Naidu, Yuvarani" sort="Naidu, Yuvarani" uniqKey="Naidu Y" first="Yuvarani" last="Naidu">Yuvarani Naidu</name></noRegion><name sortKey="Idris, Abu Seman" sort="Idris, Abu Seman" uniqKey="Idris A" first="Abu Seman" last="Idris">Abu Seman Idris</name><name sortKey="Siddiqui, Yasmeen" sort="Siddiqui, Yasmeen" uniqKey="Siddiqui Y" first="Yasmeen" last="Siddiqui">Yasmeen Siddiqui</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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